Fe-MOGs-based enzyme mimetic and its mediated electrochemiluminescence for in situ detection of H2O2 released from Hela cells

Enzyme mimetics have attracted wide interest due to their inherent enzyme-like activity and unique physicochemical properties, as well promising applications in disease diagnosis, treatment monitoring. Inspired by the attributes of nonheme iron enzymes, synthetic models were designed mimic capability investigate catalytic mechanisms. Herein, metal-organic gels (Fe-MOGs) with horseradish peroxidase (HRP) like Fe-NX structure successfully synthesized though coordination between 1,10-phenanthroline-2,9-dicarboxylic acid (PDA) exhibited excellent peroxidase-like activity. Its structure-activity relationship in-situ electrochemiluminescence (ECL) detection H2O2 secreted Hela cells further investigated. The highly dispersed active sites inside Fe-MOGs able catalyze decomposition into large amounts reactive oxygen species (ROS) via a Fenton-like reaction under low overpotential. Due accumulation ROS free radicals, luminol ECL emission was significantly amplified. A proof-of-concept biosensor constructed limit 2.2 nM linear range from 0.01 40 μM. As novel metal organic based enzyme mimetic, show great promises early cancer pathological process